Before we started the project, we did 2 punnet squares to make a hypothesis on the genetic outcome. Female fruit flies can lay hundreds of fertilised eggs during their short life span, which allows for larger populations that give easy and reliable statistical analysis. Place the flies in vials containing culture medium food 3. We did this so that we could see by their offspring which gene is dominant and which is recessive. From study of its life cycle, we are able to perform this experiment.
Agriculture, Ecosystem, Fruit 911 Words 3 Pages Lab Report: Toothpickase and Enzymes September 19, 2012 Olivia DePhillips Lab Partner: Joy Morgan Meyers Signature: Introduction Enzymes are biological molecules that catalyze chemical reactions. This lab also shows why sand is put on icy roads to increase the amount of friction between the two surfaces. As will become clear from the sample lessons explained in the next section, there are two important advantages for using Drosophila in classrooms, in particular 1 the breadth and depth of conceptual understanding of biology in the fly, and 2 the fact that flies are uniquely suited for live experiments in schools. Sexing via the sex comb can also be done successfully in the pupal stage Hadden and Cunningham, 1970. You must observe and analyze these as part of the lab. This making easier for us to differentiate them especially in the experiment about sex-linkage.
In addition I have learned how to conduct a fruit fly experiment by observing the F1 generation and interbreeding them to yield an F2 generation. The eggs hatch within 24 and on day 1 we have first instar larvae, day 2 are second instar larvae and day 3 are third instar larvae. The higher the temperature, the faster the generation time, whereas a lower to 18 degrees C temperature causes a longer generation time. Phthalic Acid melted just 20°C less than the standard melting point for Phthalic Acid. Mendel studied basic inheritance patterns and gene expression using pea plants. Thse eggs will hatch and larvae will emerge.
© 2006 fivenavy3 Our project is mainly learning about genetics. Using polymerase chain reaction… 1790 Words 8 Pages Drosophila melanogaster, often referred to as the fruit fly, is an interesting and dynamic insect that provides humanity with important information in the study of genetics. Sexing flies is critical when making crosses, so be sure student are confident in identifying the difference between the sexes. If this is still not clear to you, get help. Because of him, fruit flies have become popular for genetic research. Play long puzzles and requirements on Parchment Geographic's ado. Parental cross Expected Genotypic Ratio Expected Phenotypic ratio F1 1:1 1:1 F2 1:1 1:1 5.
We found that the surface area of the block did not change the coefficient of kinetic friction, while the types of materialsin contact were directly related to the coefficient of kinetic friction. A contributing geneticist named Gregor Mendel 1822-1884 , discovered through. Some flies have different wings, others have different eyes. We began to learn about genetics and inheritance by seeing how our flies had autosomal traits because our flies' phenotypes proved to have nothing to do with the sex of the flies. Titration is a method usedin. Although we did not get what we expected for our F2 generation we concluded that the inheritance patterns did not regulate the correct observed and expected results. With this data, a chi-square analysis was conducted.
These adult flies were anesthetized and the phenotypes sex and eye color were observed and recorded. A simple 5-10 minute colour reaction experiment demonstrating the genetics of enzyme activity. Realize many science experiments cannot be conducted and concluded within one or two lab sessions National standards covered in these lessons: Content: 1. In addition, males have tarsal sex combs on their first pair of legs. After sexing the flies, placing them in media, and letting them cross breed, we analyzed and recorded the phenotypes that were observed. For one they are very easy to maintain. Due to its small size, ease of culture and short generation time, geneticists have been using Drosophila ever since.
In some cases, the larvae can develop on normal Drosophila lab medium, but the female will. The total number of every F2 phenotype is counted. Freshwater, Hypothesis, Julius Richard Petri 1318 Words 5 Pages March 2013 Genetic Predictions in the Fruit Fly The Drosophila melanogaster is a fruit fly with a very short life cycle. Ether or Fly nap is dropped on the cotton which placed under the etherizer cap and closed the bottle for a few seconds until the ether gas fulfill the entire bottle. This lesson offers excellent opportunities to achieve differentiation and to discuss the social relevance of alcohol and alcohol abuse. At this moment, all the parental Drosophila has to be discarded to prevent mixed-up with the F2 generation. Items identified in House Report 110—258, which include: Aerial Application.
They can be winged or wingless, and have red eyes or white eyes. This is led to be by one trait being dominant over another trait. The phenotypes of the progeny are shown below. By using the appropriate symbols a diagram which shows the genotypes in each crosses, from the parental stage to the F2 stage is draw. Research of these flies initially entered labs 100 years ago.
The generation takes about 10-15 days to be complete depending on the temperature. They can be winged or wingless, and have red eyes or white eyes. To design genetic cross to illustrate segregation, independent assortment and sex linkage. One requires no anesthetizing but quick hands. For our 10th grade science class, we decided to do experiments with fruit fly genetics. We used friut flies because they have a shorter maturation period than other species and we can breed several generations within a few weeks. For the given outcomes of the genes if predicted genes were autosomal than the reciprocal crosses would generate a same F1 phenotype because the genes are carried on genes that do not distinguish based on sex.
However if we determine the mass of each element in the compound we will be able to get the true chemical formula. How to Raise Fruit Flies 1. It took nearly two weeks for adult flies in the F1 to emerge in the colder environment. The gene for this trait is located on the fruit fly's third chromosome. Similarly, the vials kept in the incubator, cross 1 and cross 2, had the best chi square values.