The Gram stain revealed it was Gram negative rods. These bacteria must be able to be identified in order to treat patients properly, efficiently and safely. The other bacteria which was microscopically viewed as gram positive was missing in the incubator, another tube of sample was derived from the lab professor. Both methyl red and Voges-Proskauer make up a combination medium. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike.
In order to do this, a nutrient agar plate was used. With a sterilized inoculating loop the colony was separately streak , inoculated and incubated. The techniques involved in this study included; what type of agar to use, inoculating, proper sterilization, gram staining and how to handle bacteria in order to avoid contamination. Gram stain showed this bacterium to be a gram-positive bacteria, it showed the blue colored rods. The medium used in this test is the Simmons Citrate Agar, which is a type of utilization media. This issue could be an indication that the streak plate could have been done more carefully or that there was a contamination of sorts on the agar plate.
This study provides students with the knowledge they need for all of these. A Gram stain was performed which identified Gram positive cocci. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown 25. Upon reviewing the identification tables, the deciding biochemical test was the Casein test which tests for the production of the enzyme casease to break down the milk protein casein. Methodology: Day One: On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of…. Blood AgarThe ability of bacteria to clump and produce hemolysin Sheep blood agar The red agar plate has shown growth and partial clearing on the side of the streak like a ring.
Virginia McDonald, Mary Thoele, Bill Salsgiver, Susie Gero. It is important to classify bacteria in order to identify them. A negative Urea test removed K. Brock Biology of Microorganisms 14th ed. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics.
Glycerol and Maltose test were then performed in hopes of confirming this bacterium. Please note that due to formatting issues the flow charts had to be removed. The unknown was identified using differential tests to determine its metabolic and morphology characteristics. Simmons Citrate Citrate slant 37°C The tube stayed green Negative The bacterium is not able to utilize citrate as its sole carbon source. Moreover, gram staining was used to understand the basic morphology of these bacteria.
The oxidase test was used to show if the organism produced cytochrome oxidase, with the addition of the oxidase reagent. Bacteria are classified on the basis of many. The following questions should be answered here: How did the test result lead to identification?. The purpose of the water is to make a pool so that the Unknown specimen can be transferred and spread in the slide. Antibiotic resistance, Bacteria, Escherichia coli 1090 Words 5 Pages The Periodic Law Lab Objectives: 1.
To enumerate the principle and methods in fermentation; and 2. After 48 hours, the Isolation 1 plate was observed. Each section must be present in a complete report. Determine the mass of the unknown solid to the nearest 0. The petri dish is then heavily inoculated with a specific bacteria and different paper disks that contain different antibiotics to examine.
To determine this we recorded the results of how the bacteria reacted to different media. Bacteria, Bacteriology, Gram staining 1749 Words 11 Pages Unknown Lab Report Unknown Organism 6 Ann Le Phuoc May 6, 2010 Dr. University of Kentucky : McGraw Hill 2 Buxton, R. Reagent B is a solution of potassium hydroxide and water. Upon returning and observing the streak plate, there was an abundance of green across the plate. There is a Phenol Red pH indicator in the solution that will show if acid was produced in fermentation. The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms 3.
Any observation of color change were recorded. The only problem I encountered was during the isolation streak, Gram positive could not be isolated on a nutrient agar plate. Observations made from the tests were then compared to a gram negative unknown chart in order to identify the bacteria. The reasons for its importance stem from the fact that microorganisms are all around us, ever changing, affecting everyone and everything they come in contact with. Materials and Methods: The unknown number 123 handed out by the Professor on March 20, 2014 contained both a gram positive bacteria and a gram negative bacteria.
Identifying bacteria… 1457 Words 6 Pages Lab Report 1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The following bacteria are negative to mannitol; Bacillus cereus, Bacillus Subtilis, ,Staphylococcus Epidermidis. The Gram stain resulted in Gram positive cocci. It was first discovered in 1885 by pediatrician and bacteriologist, Theodor Escherich 2. Seeing that no negative Gram stains were observed, a different approach was taken.